Dr Geneviève Dupont

Senior Research Associate F.R.S. - FNRS

Unité de Chronobiologie Théorique
Faculté des Sciences
Campus Plaine, C.P. 231
Université Libre de Bruxelles
1050 Brussels, Belgium

email: gdupontulb.ac.be
Phone: (32) 02 6505794
Fax: (32) 02 6505767

Picture of G. Dupont



Education

  • Licence en Chimie, Université Libre de Bruxelles, 1988
  • PhD, Université Libre de Bruxelles, 1993
  • Agrégation de l'enseignement supérieur, 2000



    Research Interests

    Computational modelling of the spatio-temporal organization of cytosolic Ca2+ signals and of their physiological implications

    In response to external stimulation, many cell types exhibit repetitive variations in their level of cytosolic Ca2+. These oscillations, initiated by an increase in inositol 1,4,5-trisphosphate (InsP3), can be ascribed to a regulation known as Ca2+-induced Ca2+ release (CICR) by which Ca2+ activates its own release from intracellular stores into the cytosol. Each Ca2+ spike is also spatially organized, as the initially localized Ca2+ increase propagates through the cell as a Ca2+ wave. These oscillations and waves possess important physiological implications as, for example, in the regulation of contraction, secretion or in the resumption of the cell cycle at fertilization. I'm working on models for the dynamics of intracellular Ca2+, in order to help uncovering the mechanism of these oscillations and waves and their physiological implications.

    I like to work in collaboration with experimentalists to analyze problems for which a computational model can help to test hypotheses and to make predictions.



    List of publications

    2014

  • Bessonard S, De Mot L, Gonze D, Barriol M, Dennis C, Goldbeter A, Dupont G and Chazaud C (2014) Gata6, Nanog, and Erk signaling control cell fate in the inner cell mass through a tristable regulatory network. Development. 141: 3637-48. doi: 10.1242/dev.109678. Epub 2014 Sep 10
  • Dupont G (2014) Models of Calcium Release. DOI 10.1007/978-1-4614-7320-6_181-3. In Encyclopedia of Computational Neuroscience (Jaeger D., Jung R. (Eds.)): Springer-Verlag Berlin Heidelberg
  • Dupont G (2014) Modeling the intracellular organization of calcium signaling. WIREs Syst. Biol. Med., doi: 10.1002/wsbm.1261.

    2013

  • De Caluwé J, Dupont G (2013) The progression towards Alzheimer's disease described as a bistable switch arising from the positive loop between amyloids and Ca2+. J. Theor. Biol. 331, 12-18.
  • Ndiaye D, Collado-Hilly M, Martin J, Prigent S, Dufour J F, Combettes L, Dupont G Characterization of the effect of the mitochondrial protein Hint2 on intracellular Ca2+. Biophys. J. (in press).
  • Tran Van Nhieu G, Liu B, Zhang J, Pierre F, Prigent S, Sansonetti P, Erneux C, Kim J, Suh P, Dupont G, Combettes L. (2013) Actin-based confinement of calcium responses during Shigella invasion. Nature Communications 4: 1567.

    2012

  • De Bock M, Wang N, Bol M, Decrock E, Ponsaerts R, De Vuyst E, Bultynck G, Dupont G, Leybaert L (2012) Connexin-43 hemichannels contribute to cytoplasmic Ca2+ oscillations by providing a bimodal Ca2+-dependent Ca2+ entry pathway. J. Biol. Chem. 287(15): 12250-66.
  • Goldbeter A, Gérard C, Gonze D, Leloup J-C, Dupont G (2012) Systems biology of cellular rhythms. FEBS Lett. (in press).
  • Gonzalez-Velez V, Gil A, Dupont G (2012) Computational methods to study Ca2+-triggered secretion at the cellular level. J. Comput. Interdisc. Sci. 3(1), 1-13.
  • Gonzalez-Velez V, Dupont G, Gil A, Gonzalez A, Quesada I (2012) Model for glucagon secretion by pancreatic alpha-cells. PlosONE 7(3): e32282.
  • Tran Van Nhieu G, Liu B, Zhang J, Pierre F, Prigent S, Sansonetti P, Erneux C, Kim J, Suh P, Dupont G, Combettes L. Actin-based confinement of calcium responses during Shigella invasion. Nature Communications (in press).

    2011

  • Abou-Lovergne A, Collado-Hilly M, Monnet F.P., Koukoui O, Prigent S, Coquil J.F. Dupont G, Combettes L (2011) Investigation of the role of sigma1-receptors in inositol 1,4,5-trisphosphate dependent calcium signaling in hepatocytes. Cell Calcium 50, 62-72.
  • Combettes L and Dupont G (2011) Approches expérimentales et de modélisation de la signalisation calcique dans les cellules. Médecine/Sciences 27, 170-176. [Abstract]
  • De Bock M, Culot M, Wang N, De Vuyst E, Decrock E, Bol M, da Costa A, Vinken M, Simon A, Rogiers V, Bultynck G, Evans W H, Dupont G, Cecchelli R, Leybaert L (2011) Connexion channels contribute to endothelial Ca2+ dynamics and are involved in endothelial permeability changes. J. Cerebr. Blood F. Met. (in press)
  • Dupont G, Loomekandja Lokenye E F and Challiss R A J (2011) A model for Ca2+ oscillations stimulated by the type 5 metabotropic glutamate receptor: An unusual mechanism based on repetitive, reversible phosphorylation of the receptor. Biochimie 93, 2132-2138.
  • Dupont G, Combettes L, Bird G, Putney J (2011) Calcium Oscillations. Cold Spring Harb Perspect Biol 1, 3. pii: a004226. [Abstract]

    2010

  • Dupont G, Croisier H (2010) Spatio-temporal organization of Ca2+ dynamics: a modelling-based approach. HSFP Journal (in press).
  • Dupont G, Heytens E, Leybaert L (2010) Oscillatory Ca2+ dynamics and cell cycle resumption at fertilization in mammals: a modelling approach. Int J Dev Biol 54, 655-65. [Abstract]

    2009

  • Dupont G, Combettes L (2009) What can we learn from the irregularity of Ca2+ oscillations? Chaos 19(3), 037112. [Abstract] [pdf file]

    2008

  • Caldara M, Dupont G, Leroy F, Goldbeter A, De Vuyst L, Cunin R (2008) Arginine biosynthesis in Escherichia coli. Experimental perturbation and mathematical modeling. J Biol Chem 283, 6347-58. [Abstract] [pdf file]
  • Dupont G, Abou-Lovergne A, Combettes L (2008) Stochastic aspects of oscillatory Ca2+ dynamics in hepatocytes. Biophys J 95, 2193-202. [Abstract] [pdf file]

    2007

  • Dupont G, Combettes L, Leybaert L (2007) Calcium dynamics : Spatio-temporal organization from the subcellular to the organ level. Int Rev Cytology 261, 193-245. [Abstract] [pdf file]
  • Vandeput F, Combettes L, Mills SJ, Backers K, Wohlkönig A, Parys JB, De Smedt H, Missiaen L, Dupont G, Potter BVL, Erneux C (2007) Biphenyl 2,3',4,5',6-pentakisphosphate, a novel inositol polyphosphate surrogate, modulates Ca2+ responses in rat hepatocytes. FASEB J 21:1481-91. [Abstract] [pdf file]

    2006

  • Dupont G and L Combettes (2006) Modelling the effect of specific inositol 1,4,5-triphosphate receptor isoforms on cellular Ca2+ signals. Biol Cell 98, 171-182. [Abstract] [pdf file]

    2004

  • Combettes L, Dupont G, and Parys JB (2004) New mechanisms and functions in Ca2+ signalling. Biol Cell 96, 1-2. [pdf file]
  • Dupont G and Dumollard R (2004) Simulation of calcium waves in ascidian eggs: Insights into the origin of the pacemaker sites and the possible nature of the sperm factor. J Cell Sci 117, 4313-4323. [Abstract] [pdf file]

    2003

  • Dupont G, Koukoui O, Clair C, Erneux C, Swillens S, and Combettes L (2003) Ca2+ oscillations in hepatocytes do not require the modulation of InsP3 3-kinase activity by Ca2+. FEBS Lett 534, 101-105. [Abstract] [pdf file]
  • Dupont G, Houart G, and De Koninck P (2003) Sensitivity of CaM kinase II to the frequency of Ca2+ oscillations: a simple model. Cell Calcium 34, 485-497. [Abstract] [pdf file]
  • Dupont G, Houart G, and Goldbeter A (2003) From simple to complex Ca2+ oscillations: Regulatory mechanisms and theoretical models. In Lectures Notes in Physics. Understanding calcium dynamics. Experiments and theory. M. Falcke and D. Malchow (eds). Springer (Berlin), pp 131-152. [Abstract]

    2002

  • Dumollard R, Carroll J, Dupont G, and Sardet C (2002) Calcium wave pacemakers in eggs. J Cell Sci 115, 3557-3564. [Abstract] [pdf file]

    2001

  • Clair C, Chalumeau C, Tordjmann T, Poggioli J, Erneux C, Dupont G, and Combettes L (2001) Investigation of the roles of Ca(2+) and InsP(3) diffusion in the coordination of Ca(2+) signals between connected hepatocytes. J Cell Sci 114, 1999-2007. [Abstract] [pdf file]
  • Goldbeter A, Gonze D, Houart G, Leloup J-C, Halloy J, and Dupont G (2001) From simple to complex oscillatory behavior in metabolic and genetic control network. Chaos 11, 247-260. [Abstract] [pdf file]

    2000

  • Dupont G, Swillens S, ClairC ,Tordjmann T, and Combettes L (2000) Hierarchical organization of calcium signals in hepatocytes: from experiments to models. Biochim Biophys Acta 1498, 134-152. [Abstract] [pdf file]
  • Dupont G, Tordjmann T, Clair C, Swillens S, Claret M, and Combettes L (2000) Mechanism of receptor-oriented intercellular calcium wave propagation in hepatocytes. FASEB J 14, 279-289. [Abstract] [pdf file]
  • Gall D, Baus E, and Dupont G (2000) Activation of the liver glycogen phosphorylase by Ca2+ oscillations: A theoretical study. J Theor Biol 207, 445-454. [Abstract] [pdf file]
  • Goldbeter A, Dupont G, and Halloy J (2000) The frequency encoding of pulsatility. Novartis Found Symp 227, 19-45. [Abstract]

    1999

  • Dupont G (1999) Spatio-temporal organization of intracellular calcium signals: From experimental to theoretical aspects. Comments on Theor Biol 5, 305-340.
  • Houart G, Dupont G, and Goldbeter A (1999) Bursting, chaos and birhythmicity originating from self-modulation of the inositol 1,4,5-triphosphate signal in a model for intracellular Ca 2+ oscillations. Bull Math Biol 61, 507-530. [Abstract] [pdf file]
  • Swillens S, Dupont G, Combettes L, and Champeil P (1999) From calcium blips to calcium puffs: Theoretical analysis of the requirements for interchannel communication. Proc Natl Acad Sci USA 96, 13750-13755. [Abstract] [pdf file]

    1998

  • Dupont G (1998) Link between fertilization-induced Ca2+ oscillations and relief from metaphase II arrest in mammalian eggs: a model based on calmodulin-dependent kinase II activation. Biophys Chem 72, 153-167. [Abstract] [pdf file]
  • Dupont G (1998) Theoretical insights into the mechanism of spiral Ca2+ wave initiation in Xenopus oocytes. Am J Physiol 275, C317-C322. [Abstract] [pdf file]
  • Dupont G and Goldbeter A (1998) CaM kinase II as frequency decoder of Ca2+ oscillations. BioEssays 20, 607-610. [Abstract]
  • Swillens S, Champeil P, Combettes L, and Dupont G (1998) Stochastic simulation of a single inositol 1,4,5-trisphosphate-sensitive Ca2+ channel reveals repetitive openings during 'blip-like' Ca2+ transients. Cell Calcium 23, 291-302. [pdf file]

    1997

  • Borghans J, Dupont G, and Goldbeter A (1997) Complex intracellular calcium oscillations: a theoretical exploration of possible mechanisms. Biophys Chem 66, 25-41. [pdf file]
  • Dupont G and Erneux T (1997) Simulations of the effects of inositol 1,3,4-triphosphate 3-kinase and 5-phosphatase activities on Ca2+ oscillations. Cell Calcium 22, 313-323.
  • Dupont G and Goldbeter A (1997) Modeling oscillations and waves of cytosolic calcium. Nonlinear Analysis, Theory, Methods and Applications 30, 1781-1792. [pdf file]

    1996

  • Dupont G, McGuinness OM, Johnson MH, Berridge MJ, and Borgese F (1996) Phospholipase C in mouse oocytes: Characterization of beta and gamma isoforms and their possible involvement in sperm-induced Ca2+ spiking. Biochem J 316, 583-591. [pdf file]
  • Dupont G and Swillens S (1996) Quantal release, incremental detection, and long-period Ca 2+ oscillations in a model based on regulatory Ca2+ -binding sites along the permeation pathway. Biophys J 71, 1714-1722. [pdf file]
  • Dupont G, Pontès J, and Goldbeter A (1996) Modeling spiral Ca2+ waves in single cardiac cells: Role of the spatial heterogeneity created by the nucleus. Am J Physiol 271, C1390-C1399.

    1994

  • Berridge MJ and Dupont G (1994) Spatial and temporal signalling by calcium. Curr Opin Cell Biol 6, 267-274.
  • Dupont G and Goldbeter A (1994) Properties of intracellular Ca2+ waves generated by a model based on Ca 2+-induced Ca2+ release. Biophys J 67, 2191-2204. [pdf file & erratum]
  • Petersen CH and Dupont G (1994) The initiation of a calcium signal in Xenopus oocytes. Cell Calcium 16, 391-403.
  • Dupont G and Goldbeter A (1994) Properties of calcium waves generated by a model based on calcium-induced calcium release. Biophys J 67, 2191-2204.

    1993

  • Dupont G and Goldbeter A (1993) One-pool model for Ca2+ oscillations involving Ca2+ and inositol 1,4,5-triphosphate as co-agonists for Ca2+ release. Cell Calcium 14, 311-322. [pdf file]
  • Dupont G, Moulinasse S, Ryckaert JP, and Bauss M (1993) The bcc-fcc-fluid triple point as obtained from Monte-Carlo simulations of the Yukawa model for monodisperse colloidal suspensions. Mol Phys 79, 453-456.
  • Goldbeter A and Dupont G (1993) Wavelike propagation of cAMP and Ca2+ signals: Link with excitability and oscillations. In: Oscillations and Morphogenesis (L Rensing, ed.), M Dekker, New York, pp. 195-209.
  • Goldbeter A, Li YX, and Dupont G (1993) Pulsatile signalling in intercellular communication: An overview of theoretical and experimental aspects. In: Proceedings of the First European Conference on Mathematics Applied to Biology and Medicine (J Demongeot & V Capasso, eds.), Wuerz Publ., Winnipeg, Canada, pp. 429-439.

    1992

  • Dupont G and Goldbeter A (1992) Oscillations and waves of cytosolic calcium: insights from theoretical models. BioEssays 14, 485-493.
  • Dupont G and Goldbeter A (1992) Protein phosphorylation driven by intracellular calcium oscillations: A kinetic analysis. Biophys Chem 42, 257-270.

    1991

  • Dupont G, Berridge MJ, and Goldbeter A (1991) Signal-induced Ca2+ oscillations: properties of a model based on Ca2+-induced Ca2+ release. Cell Calcium 12, 73-85.
  • Goldbeter A and Dupont G (1991) Phosphorylation and the frequency encoding of signal-induced calcium oscillations. In: Cellular Regulation by Protein Phosphorylation (L Heilmeyer, ed.) Springer, Berlin, pp. 35-39.
  • Goldbeter A, Li YX, and Dupont G (1991) Periodicity and chaos in cAMP, hormonal and calcium signalling. In: Complex Dynamics and Biological Evolution (E Mosekilde, ed.), Plenum Press, New York, pp. 131-143.

    1990

  • Dupont G, Berridge MJ, and Goldbeter A (1990) Latency correlates with period in a model for signal-induced Ca2+ oscillations based on Ca2+-induced Ca2+ release. Cell Regul 1, 853-861. [pdf file]
  • Goldbeter A, and Dupont G (1990) Allosteric regulation, cooperativity, and biochemical oscillations. Biophys Chem 37, 341-353.
  • Goldbeter A, Dupont G, and Berridge MJ (1990) Minimal model for signal-induced Ca2+ oscillations and for their frequency encoding through protein phosphorylation. Proc Natl Acad Sci USA 87, 1461-1465.
  • Goldbeter A, Li Y, and Dupont G (1990) Oscillatory dynamics in intercellular communication. Biomed Biochim Acta 49, 935-940.

    1989

  • Dupont G and Goldbeter A (1989) Theoretical insights into the origin of signal-induced calcium oscillations. In: Cell to Cell Signalling: From Experiments to Theoretical Models (A Goldbeter, ed.) Academic Press, London, pp. 461-474.
  • Nicolis G, Amellal A, Dupont G, and Mareschal M (1989) Nonequilibrium states and long range correlations in chemical dynamics. Journal of Molecular Liquids 41, 5-19.


    ULB - UTC: G. Dupont / Revised :